COMPARATIVE ESTIMATION OF LEVEL OF TNF- ? IN GINGIVAL CREVICULAR FLUID AND BLOOD SERUM IN PATIENT WITH CHRONIC PERIODONTITIS BEFORE AND AFTER SCALING AND ROOT PLANING: A BIOCHEMICAL STUDY

Abstract

TNF-? is produced by various cells and is involved in normal inflammatory and immune responses. TNF-? is secreted by macrophages, monocytes, neutrophils and T-cells.TNF is also a potent trigger for cellular apoptosis, or programmed cell death. Thus, TNF is critical to wound debridement either under septic or aseptic circumstances. TNF- ? induces bone resorption but it is much less potent.
Aim-The Aim of the present study is to Estimate the level of TNF -? in Gingival Crevicular Fluid and Blood Serum in patient with Chronic Periodontitis before and after Scaling and Root Planing (SRP).
Materials and Methods-25 subjects within age range of 25-65 years both male and female were selected on basis of inclusion criteria and were categorized into two treatment groups.
GROUP I (n=25): Chronic Generalized Periodontitis patients before scaling and root planing
GROUP II (n=25): Chronic Generalized Periodontitis (group1) patients after one month of scaling and root planing. Biochemical analysis of GCF and serum samples were done to estimate the level of TNF- ? at baseline and after 1 month of SRP using ELISA kit (BOSTER IMMUNOLEADER HUMAN TNF? ELISA KIT).
Results-Showed elevated levels of TNF-a in group I subjects as compared to Group II subjectsin both GCF and serum and showed an association between periodontal disease and levels of TNF- ?.
Conclusion-Increase in level of cytokines, includingTNF-? and IL-6 has been seen in close association with periodontal disease.Level of cytokines before and after periodontal therapy reflects the response to periodontal treatment.Clinical parameters and level of TNF-? decreased after scaling and root planing. These observations indicates positive association between periodontal disease and increased level of TNF-? in GCF and Serum.