To probe the effect of Resveratrol on caspase - 3 & caspase -9 activity in Human Papilloma Virus positive Ca Ski cell-line in 48 hours cell cultures

Abstract

Objectives:  Cervical cancer is the leading cause of cancer mortality, accounting for a significant percentage of all cancer deaths amongst women. Resveratrol(3,5,4-trihydroxystilbene),a natural pytoalexin ,belongs to a class of  polyphenolic compounds called stilbenes. The rich natural  sources of resveratrol are grapes,nuts,red wine and roots and stalks of Japanese knotweed(polygonum cuspidatum).It has potent antioxidant,anti-aging,anti-cancer and anti-inflammatory properties .  As it was demonstrated in a previous study that Resveratrol causes inhibition of cancer cell proliferation in a dose and tiume dependent manner using cell viability assays , our  objective was to probe further that Resveratrol treated cells have augmented caspase -3 and caspase - 9 activites than the untreated  Ca Ski cells .

Methods: Caspase -3 and Caspase-9 activity levels were  measured in Resveratrol(90µg/ml) treated and untreated cells that were taken as control using the colorimetric substrates DEVD-Pna and absorbance was read at 405nm to demonstrate augmentation of caspase 3 & 9 activities and GPx activity in Resveratrol treated cells in 48 hours culture.

Results & conclusion: Resveratrol treated Ca Ski cell lines that were cultured for 48 hrs showed high levels of caspase-3 and caspase-9 thereby indicating that Resveratrol has an apoptotic and anti-proliferative effect on Ca Ski cells.

Keywords: Resveratrol, Cervical cancer, caspase -3 , caspase- 9