ANTIFEEDANT ACTIVITY OF NANOFORMULATIONS OF CLEOME VISCOSA, AGAINST PEST- TRIBOLIUM CASTANEUM USING HPLC
Abstract
Background: Now many agricultural engineers are seeking knowledge of nano-materials for engineering bio-pesticides, fertilizer coatings and other purposes. These Nanoparticles can be exploited as they offer a greater surface area and circulate more easily.
Materials & Methods: Insecticidal activity of C. viscosa Column fractions against stored product pests. Antifeedant Bioassay was done by fumigation method. Separation and fractionation was done by Column chromatography. TEM morphology of nano-sio2 particle was performed on transmission electron micrograph. Statistical analysis: The average larval mortality data were subjected to probit analysis for calculating lethal concentrations LC50 and LC95 and other statistics at 95% fiducial limits of upper confidence limit and lower confidence limit were calculated.
Results: The extracts of C. viscose was moderately toxic and needed at least 2 mg/ ml to obtain 50% mortality. C. viscosa showed high toxicity with a LC50 of 0.8 mg/ml and LC95 of 1.95 mg/ml. Thus it can be concluded that the terpenes adsorbed on the silica through strong hydrogen bonding. All the results above further illustrate that adsorption of terpene has an added advantage that in spite of less agglutination it also exhibit the high dispersion stability.
Conclusion: Among the tested plant materials, C. viscosa proved as highly toxic against stored product pests as well as mosquito larvae. This active fraction will be further processed for the pure compound isolation, identification for the principle bioactive compound. The two principle bioactive compounds showed enhanced antifeedant activity against agricultural pests on Nanoformulations.
Keywords: Nanopesticide, Cleome viscosa, Tribolium castaneum, FT-IR analysis, HLPC
