Distribution of ANA Pattern and Autoantibody Profile in Various Autoimmune Disorders -A Tertiary Care Centre Experience.

Abstract

Background: Type of antibodies produced by an individual in response to any autoimmune disorder varies from person to person and from place to place depending upon the immune status of the patient.

Aims & objectives:  

To describe the fluorescence pattern of ANA by indirect immunofluorescent assay in patients with autoimmune diseases and compare it with their ANA profile.

Material & Methods:

In this retrospective study (January 2018 to December 2020) we reviewed the case records of 96 patients with different autoimmune diseases. Their demographic details like age, gender, family history, presenting symptoms were tabulated. We compared their respective ANA test reports (Pattern and profile)

Results:  Data 96 patients (20 male and 76 females) was analyzed. The age range of the study cohort was 10- 77 years, mean age was 39.53. There were 23(23.9%) patients with SLE, 34(35.4%) with MCTD. 6 (6.2%) Sjogren's syndrome. Glomerulonephritis was present in 7(7.2%) patients and Rheumatoid arthritis in 4 (4.1%) . All study participants had ANA positive and ANA pattern was seen as homogenous in 34(35.4%), speckled 45(46%), nuclear pattern was seen in 33(34.9%) . LIA was found to be significantly positive in sjogren's disease while in SLE only 22 patients had positive LIA. None of our Rheumatoid arthritis patients had positive LIA  

Conclusion:  We conclude that it is better to restrict performing line immunoassays as these are expensive and use ANA-IIF fluorescent patterns to predict presence of autoantibodies to diagnose an autoimmune disease.